Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probing.

@article{Subczynski2007ThreedimensionalDS,
  title={Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probing.},
  author={Witold K Subczynski and A. E. Wiśniewska and James S. Hyde and Akihiro Kusumi},
  journal={Biophysical journal},
  year={2007},
  volume={92 5},
  pages={1573-84}
}
Membranes made of dimyristoylphosphatidylcholine and cholesterol, one of the simplest paradigms for the study of liquid ordered-disordered phase separation, were investigated using a pulse-EPR spin-labeling method in which bimolecular collision of molecular oxygen with the nitroxide spin label is measured. This method allowed discrimination of liquid-ordered, liquid-disordered, and solid-ordered domains because the collision rates (OTP) differ in these domains. Furthermore, the oxygen transport… CONTINUE READING

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