Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers.

@article{IshaugRiley1998ThreedimensionalCO,
  title={Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers.},
  author={S L Ishaug-Riley and G M Crane-Kruger and Michael J. Yaszemski and Antonios G Mikos},
  journal={Biomaterials},
  year={1998},
  volume={19 15},
  pages={1405-12}
}
Neonatal rat calvarial osteoblasts were cultured in 90% porous, 75:25 poly(DL-lactic-co-glycolic acid) (PLGA) foam scaffolds for up to 56 days to examine the effects of the cell seeding density, scaffold pore size, and foam thickness on the proliferation and function of the cells in this three-dimensional environment. Osteoblasts were seeded at either 11.1 x 10(5) or 22.1 x 10(5) cells per cm2 onto PLGA scaffolds having pore sizes in the range of 150-300 or 500-710 microm with a thickness of… CONTINUE READING