Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.

@article{Smith2011ThreedimensionalCO,
  title={Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.},
  author={Barry H. Smith and Lawrence S. Gazda and Bryan L Conn and Kanti Jain and Shirin Asina and Daniel M. Levine and Thomas S. Parker and Melissa A. Laramore and Prithy C Martis and Horatiu V Vinerean and Eric M David and Suizhen Qiu and Carlos Cordon-Cardo and Richard Delmar Hall and Bruce R. Gordon and Carolyn H Diehl and Kurt H. Stenzel and Albert L. Rubin},
  journal={Cancer research},
  year={2011},
  volume={71 3},
  pages={
          716-24
        }
}
The culture of tumor cell lines in three-dimensional scaffolds is considered to more closely replicate the in vivo tumor microenvironment than the standard method of two-dimensional cell culture. We hypothesized that our method of encapsulating and maintaining viable and functional pancreatic islets in agarose-agarose macrobeads (diameter 6-8 mm) might provide a novel method for the culture of tumor cell lines. In this report we describe and characterize tumor colonies that form within… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 32 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 42 REFERENCES