Three-dimensional cellular ultrastructure resolved by X-ray microscopy

  title={Three-dimensional cellular ultrastructure resolved by X-ray microscopy},
  author={Gerd Schneider and Peter Guttmann and Stefan Heim and Stefan Rehbein and F. M{\"u}ller and Kan Nagashima and J Bernard Heymann and Waltraud G. M{\"u}ller and James G. McNally},
  journal={Nature Methods},
We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ∼36-nm (Rayleigh) and ∼70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear… CONTINUE READING
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N at u re A m er ic a, In c. A ll ri g h ts r es

  • G. Schneider
  • Ultramicroscopy 75,
  • 1998

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