Three-dimensional cellular ultrastructure resolved by X-ray microscopy

Abstract

We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ∼36-nm (Rayleigh) and ∼70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.

DOI: 10.1038/nmeth.1533
02040602011201220132014201520162017
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@article{Schneider2010ThreedimensionalCU, title={Three-dimensional cellular ultrastructure resolved by X-ray microscopy}, author={Gerd Schneider and Peter Guttmann and Stefan Heim and Stefan Rehbein and Florian Mueller and Kunio Nagashima and J Bernard Heymann and Waltraud G. M{\"{u}ller and James G. McNally}, journal={Nature Methods}, year={2010}, volume={7}, pages={985-987} }