Three charged amino acids in extracellular loop 1 are involved in maintaining the outer pore architecture of CFTR

@inproceedings{Cui2014ThreeCA,
  title={Three charged amino acids in extracellular loop 1 are involved in maintaining the outer pore architecture of CFTR},
  author={Guiying Cui and Kazi Shefaet Rahman and Daniel T. Infield and Christopher Kuang and Chengyu Z. Prince and Nael A. McCarty},
  booktitle={The Journal of general physiology},
  year={2014}
}
The cystic fibrosis (CF) transmembrane conductance regulator (CFTR) bears six extracellular loops (ECL1-6); ECL1 is the site of several mutations associated with CF. Mutation R117H has been reported to reduce current amplitude, whereas D110H, E116K, and R117C/L/P may impair channel stability. We hypothesized that these amino acids might not be directly involved in ion conduction and permeation but may contribute to stabilizing the outer vestibule architecture in CFTR. We used cRNA injected… CONTINUE READING
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