Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production

@article{Stahmann2000ThreeBP,
  title={Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production},
  author={Klaus-Peter Stahmann and Jos{\'e} Luis Revuelta and Harald Seulberger},
  journal={Applied Microbiology and Biotechnology},
  year={2000},
  volume={53},
  pages={509-516}
}
Abstract Chemical riboflavin production, successfully used for decades, is in the course of being replaced by microbial processes. These promise to save half the costs, reduce waste and energy requirements, and use renewable resources like sugar or plant oil. Three microorganisms are currently in use for industrial riboflavin production. The hemiascomycetes Ashbya gossypii, a filamentous fungus, and Candida famata, a yeast, are naturally occurring overproducers of this vitamin. To obtain… 
Molecular Studies of the Flavinogenic Fungus Ashbya gossypii and the Flavinogenic Yeast Candida famata
TLDR
Development of molecular tools for studying A. gossypii and C. famata are described and examples of successful utilization of these approaches for construction of the even more effective riboflavin producers are described.
Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida Famata.
TLDR
It is shown that the activation of metabolic flux toward purine nucleotide biosynthesis is promising approach to improve riboflavin production.
Microbial production of riboflavin using riboflavin overproducers,Ashbya gossypii, Bacillus subtilis, andCandida famate: An overview
TLDR
With respect to improved riboflavin production, the supplementation of GTP, a process-limiting precursor must be considered, and the development of pathways to strengthen GTP supplementation using biotechnological techniques remains an issue for future research.
Strategies to Increase the Production of Biosynthetic Riboflavin
TLDR
Fermentation increases the yield of riboflavin using genetic engineering technology to modify and induce rib oflavin production in the strain, as well as to regulate the metabolic flux of the purine pathway and pentose phosphate pathway (PP pathway), thereby optimizing the culture process.
Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida famata.
TLDR
Yeast homologs of mammal BCRP gene encoding putative rib oflavin efflux protein (excretase) were identified in the flavinogenic yeasts Debaryomyces hansenii and C. famata and may be useful to increase the riboflavin yield in a ribofavin production process using a recombinant overproducing C.famata strain or other Flavinogenic microorganisms.
Production and Molecular Characterization of riboflavingenic Strain of Aashbya gossypii 1
TLDR
Ashbya gossypii has an ability to overproduce riboflavin and it can be used in large scale production with expectation of significant amounts and the best incubation period was 18 days and suitable pH was 6.5 which enhance production of rib oflavin.
Lactose Inducible Expression of Transcription Factor Gene SEF1 Increases Riboflavin Production in the Yeast Candida famata
TLDR
The constructed strains containing additional copy of SEF1 gene under the control of LAC4 promoter is a perfect platform for development of industrial riboflavin production on by-product of dairy industry, whey.
Engineering Ashbya Gossypii for the Production of Ricinoleic and Linoleic Acid
TLDR
This strain presents an increased lipid accumulation with respect to wild type as this desaturation step is one of the rate limiting steps in lipid synthesis, representing a great starting point for metabolic engineering of this fungus for producing omega-6 and omega-3 fatty acids.
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