Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production

@article{Stahmann2000ThreeBP,
  title={Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production},
  author={K. Stahmann and J. Revuelta and H. Seulberger},
  journal={Applied Microbiology and Biotechnology},
  year={2000},
  volume={53},
  pages={509-516}
}
Abstract Chemical riboflavin production, successfully used for decades, is in the course of being replaced by microbial processes. These promise to save half the costs, reduce waste and energy requirements, and use renewable resources like sugar or plant oil. Three microorganisms are currently in use for industrial riboflavin production. The hemiascomycetes Ashbya gossypii, a filamentous fungus, and Candida famata, a yeast, are naturally occurring overproducers of this vitamin. To obtain… Expand
Molecular Studies of the Flavinogenic Fungus Ashbya gossypii and the Flavinogenic Yeast Candida famata
TLDR
Development of molecular tools for studying A. gossypii and C. famata are described and examples of successful utilization of these approaches for construction of the even more effective riboflavin producers are described. Expand
Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida Famata.
TLDR
It is shown that the activation of metabolic flux toward purine nucleotide biosynthesis is promising approach to improve riboflavin production. Expand
Microbial production of riboflavin using riboflavin overproducers,Ashbya gossypii, Bacillus subtilis, andCandida famate: An overview
TLDR
With respect to improved riboflavin production, the supplementation of GTP, a process-limiting precursor must be considered, and the development of pathways to strengthen GTP supplementation using biotechnological techniques remains an issue for future research. Expand
Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications.
TLDR
The biotechnological potential of A. gossypii beyond riboflavin production is addressed by presenting a physiological and metabolic perspective over this fungus, and the molecular toolbox available for its manipulation is presented. Expand
Biotechnological Strategies of Riboflavin Biosynthesis in Microbes
TLDR
An updated understanding of riboflavin biosynthesis is provided and can promote the research and development of fermented food products rich in rib oflavin. Expand
Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida famata.
TLDR
Yeast homologs of mammal BCRP gene encoding putative rib oflavin efflux protein (excretase) were identified in the flavinogenic yeasts Debaryomyces hansenii and C. famata and may be useful to increase the riboflavin yield in a ribofavin production process using a recombinant overproducing C.famata strain or other Flavinogenic microorganisms. Expand
Strategies to Increase the Production of Biosynthetic Riboflavin
TLDR
Fermentation increases the yield of riboflavin using genetic engineering technology to modify and induce rib oflavin production in the strain, as well as to regulate the metabolic flux of the purine pathway and pentose phosphate pathway (PP pathway), thereby optimizing the culture process. Expand
Production and Molecular Characterization of riboflavingenic Strain of Aashbya gossypii 1
Riboflavin, a yellow, water soluble solid, is wide distributed in plants and animals, and plays an important role in live organisms because it is the precursor of flavin mononucleotide (riboflavinExpand
Engineering Ashbya Gossypii for the Production of Ricinoleic and Linoleic Acid
TLDR
This strain presents an increased lipid accumulation with respect to wild type as this desaturation step is one of the rate limiting steps in lipid synthesis, representing a great starting point for metabolic engineering of this fungus for producing omega-6 and omega-3 fatty acids. Expand
Engineering Ashbya gossypii strains for de novo lipid production using industrial by‐products
TLDR
By altering the fatty acyl‐CoA pool and manipulating the regulation of the main ∆9 desaturase gene, A. gossypii strains with significantly increased (up to fourfold) de novo lipid biosynthesis using glucose as the only carbon source in the fermentation broth are obtained. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 54 REFERENCES
Correlation of isocitrate lyase activity and riboflavin formation in the riboflavin overproducer Ashbya gossypii.
TLDR
The idea of an ICL bottleneck in the riboflavin overproducer A. gossypii when plant oil is used as the substrate is supported. Expand
Threonine Aldolase Overexpression plus Threonine Supplementation Enhanced Riboflavin Production inAshbya gossypii
TLDR
An improvement of riboflavin production in this fungus is reported by overexpression of the glycine biosynthetic enzyme threonine aldolase, which led to a strong enhancement, which could not be achieved by supplementation with glycine alone, and was attributed to an almost quantitative uptake ofThreonine and its intracellular conversion into glycine. Expand
Biosynthesis of Riboflavin, Biotin, Folic Acid, and Cobalamin
TLDR
The chapter discusses the organization and regulation of the riboflavin biosynthetic genes and presents results from laboratory on how this information can be used to enhance vitamin production by B. subtilis. Expand
Regulation of Riboflavin Biosynthesis inBacillus subtilis Is Affected by the Activity of the Flavokinase/Flavin Adenine Dinucleotide Synthetase Encoded byribC
TLDR
It is shown that the ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase (FAD-synthetase) activities, which indicates that flavin nucleotides, but not riboflavin, have an effector function for regulation of rib oflavin biosynthesis in B. subtilis. Expand
Inhibition of purified isocitrate lyase identified itaconate and oxalate as potential antimetabolites for the riboflavin overproducer Ashbya gossypii.
TLDR
A specific isocitrate lyase (ICL) activity was detected in cultures of the riboflavin-producing fungus Ashbya gossypii during growth on soybean oil and showed a strong inhibition and may therefore be interesting as antimetabolites. Expand
Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis
TLDR
Estimation of intracellular metabolic fluxes by a refined mass balance approach revealed a substantial, growth rate-dependent flux through the oxidative branch of the pentose phosphate pathway, indicated to be increased in the strain engineered for riboflavin formation. Expand
Genetic engineering of Bacillus subtilis for the commercial production of riboflavin
Recombinant DNA engineering was combined with mutant selection and fermentation improvement to develop a strain of Bacillus subtilis that produces commercially attractive levels of riboflavin. The B.Expand
Physiological Consequence of Disruption of the VMA1Gene in the Riboflavin Overproducer Ashbya gossypii *
The vacuolar ATPase subunit A structural geneVMA1 of the biotechnologically important riboflavin overproducer Ashbya gossypii was cloned and disrupted to prevent riboflavin retention in the vacuolarExpand
Regulation and properties of a fungal lipase showing interfacial inactivation by gas bubbles, or droplets of lipid or fatty acid.
TLDR
Growth experiments performed under lipase-stabilizing conditions revealed a negative influence of glucose, glycerol or oleic acid on detectable lipase activity, probably due to a regulation of lipase formation. Expand
Presence of Escherichia coli of a deaminase and a reductase involved in biosynthesis of riboflavin
Two enzymes have been partially purified from extracts of Escherchia coli B which together catalyze the conversion of the product of the action of GTP cyclohydrolase II,Expand
...
1
2
3
4
5
...