Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production

  title={Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production},
  author={Klaus-Peter Stahmann and Jos{\'e} Luis Revuelta and Harald Seulberger},
  journal={Applied Microbiology and Biotechnology},
Abstract Chemical riboflavin production, successfully used for decades, is in the course of being replaced by microbial processes. These promise to save half the costs, reduce waste and energy requirements, and use renewable resources like sugar or plant oil. Three microorganisms are currently in use for industrial riboflavin production. The hemiascomycetes Ashbya gossypii, a filamentous fungus, and Candida famata, a yeast, are naturally occurring overproducers of this vitamin. To obtain… 
Molecular Studies of the Flavinogenic Fungus Ashbya gossypii and the Flavinogenic Yeast Candida famata
Development of molecular tools for studying A. gossypii and C. famata are described and examples of successful utilization of these approaches for construction of the even more effective riboflavin producers are described.
Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida Famata.
It is shown that the activation of metabolic flux toward purine nucleotide biosynthesis is promising approach to improve riboflavin production.
Microbial production of riboflavin using riboflavin overproducers,Ashbya gossypii, Bacillus subtilis, andCandida famate: An overview
With respect to improved riboflavin production, the supplementation of GTP, a process-limiting precursor must be considered, and the development of pathways to strengthen GTP supplementation using biotechnological techniques remains an issue for future research.
Strategies to Increase the Production of Biosynthetic Riboflavin
Fermentation increases the yield of riboflavin using genetic engineering technology to modify and induce rib oflavin production in the strain, as well as to regulate the metabolic flux of the purine pathway and pentose phosphate pathway (PP pathway), thereby optimizing the culture process.
Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida famata.
Yeast homologs of mammal BCRP gene encoding putative rib oflavin efflux protein (excretase) were identified in the flavinogenic yeasts Debaryomyces hansenii and C. famata and may be useful to increase the riboflavin yield in a ribofavin production process using a recombinant overproducing C.famata strain or other Flavinogenic microorganisms.
Production and Molecular Characterization of riboflavingenic Strain of Aashbya gossypii 1
Ashbya gossypii has an ability to overproduce riboflavin and it can be used in large scale production with expectation of significant amounts and the best incubation period was 18 days and suitable pH was 6.5 which enhance production of rib oflavin.
Lactose Inducible Expression of Transcription Factor Gene SEF1 Increases Riboflavin Production in the Yeast Candida famata
The constructed strains containing additional copy of SEF1 gene under the control of LAC4 promoter is a perfect platform for development of industrial riboflavin production on by-product of dairy industry, whey.
Engineering Ashbya Gossypii for the Production of Ricinoleic and Linoleic Acid
This strain presents an increased lipid accumulation with respect to wild type as this desaturation step is one of the rate limiting steps in lipid synthesis, representing a great starting point for metabolic engineering of this fungus for producing omega-6 and omega-3 fatty acids.


Correlation of isocitrate lyase activity and riboflavin formation in the riboflavin overproducer Ashbya gossypii.
The idea of an ICL bottleneck in the riboflavin overproducer A. gossypii when plant oil is used as the substrate is supported.
Threonine Aldolase Overexpression plus Threonine Supplementation Enhanced Riboflavin Production inAshbya gossypii
An improvement of riboflavin production in this fungus is reported by overexpression of the glycine biosynthetic enzyme threonine aldolase, which led to a strong enhancement, which could not be achieved by supplementation with glycine alone, and was attributed to an almost quantitative uptake ofThreonine and its intracellular conversion into glycine.
Biosynthesis of Riboflavin, Biotin, Folic Acid, and Cobalamin
The chapter discusses the organization and regulation of the riboflavin biosynthetic genes and presents results from laboratory on how this information can be used to enhance vitamin production by B. subtilis.
Regulation of Riboflavin Biosynthesis inBacillus subtilis Is Affected by the Activity of the Flavokinase/Flavin Adenine Dinucleotide Synthetase Encoded byribC
It is shown that the ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase (FAD-synthetase) activities, which indicates that flavin nucleotides, but not riboflavin, have an effector function for regulation of rib oflavin biosynthesis in B. subtilis.
Inhibition of purified isocitrate lyase identified itaconate and oxalate as potential antimetabolites for the riboflavin overproducer Ashbya gossypii.
A specific isocitrate lyase (ICL) activity was detected in cultures of the riboflavin-producing fungus Ashbya gossypii during growth on soybean oil and showed a strong inhibition and may therefore be interesting as antimetabolites.
Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis
Estimation of intracellular metabolic fluxes by a refined mass balance approach revealed a substantial, growth rate-dependent flux through the oxidative branch of the pentose phosphate pathway, indicated to be increased in the strain engineered for riboflavin formation.
Genetic engineering of Bacillus subtilis for the commercial production of riboflavin
Recombinant DNA engineering was combined with mutant selection and fermentation improvement to develop a strain of Bacillus subtilis that produces commercially attractive levels of riboflavin. The B.
Physiological Consequence of Disruption of the VMA1Gene in the Riboflavin Overproducer Ashbya gossypii *
The vacuolar ATPase subunit A structural geneVMA1 of the biotechnologically important riboflavin overproducer Ashbya gossypii was cloned and disrupted to prevent riboflavin retention in the vacuolar
Regulation and properties of a fungal lipase showing interfacial inactivation by gas bubbles, or droplets of lipid or fatty acid.
Growth experiments performed under lipase-stabilizing conditions revealed a negative influence of glucose, glycerol or oleic acid on detectable lipase activity, probably due to a regulation of lipase formation.