Three Isoforms of Mammalian Hyaluronan Synthases Have Distinct Enzymatic Properties*

  title={Three Isoforms of Mammalian Hyaluronan Synthases Have Distinct Enzymatic Properties*},
  author={N. Itano and T. Sawai and M. Yoshida and P. Lenas and Y. Yamada and M. Imagawa and T. Shinomura and M. Hamaguchi and Y. Yoshida and Y. Ohnuki and S. Miyauchi and A. Spicer and J. A. Mcdonald and K. Kimata},
  journal={The Journal of Biological Chemistry},
  pages={25085 - 25092}
  • N. Itano, T. Sawai, +11 authors K. Kimata
  • Published 1999
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • Three mammalian hyaluronan synthase genes,HAS1, HAS2, and HAS3, have recently been cloned. In this study, we characterized and compared the enzymatic properties of these three HAS proteins. Expression of any of these genes in COS-1 cells or rat 3Y1 fibroblasts yielded de novoformation of a hyaluronan coat. The pericellular coats formed by HAS1 transfectants were significantly smaller than those formed by HAS2 or HAS3 transfectants. Kinetic studies of these enzymes in the membrane fractions… CONTINUE READING
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