Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution

@article{Chiang2011ThreeDimensionalRO,
  title={Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution},
  author={Ann‐Shyn Chiang and Chih-Yung Lin and Chao-Chun Chuang and Hsiu-Ming Chang and Chang-Huain Hsieh and Chang-Wei Yeh and Chi-Tin Shih and Jian-Jheng Wu and Guo-Tzau Wang and Yung-Chang Chen and Cheng-Chi Wu and Guan-Yu Chen and Yu-Tai Ching and Ping-Chang Lee and Chih-Yang Lin and Hui-Hao Lin and Chia-Chou Wu and Hao-Wei Hsu and Yun-An Huang and Jing-Yi Chen and Hsin-Jung Chiang and Chun-Fang Lu and Ru-Fen Ni and Chao-yuan Yeh and Jenn-Kang Hwang},
  journal={Current Biology},
  year={2011},
  volume={21},
  pages={1-11}
}

Figures from this paper

A mesoscale connectome of the mouse brain
TLDR
A brain-wide, cellular-level, mesoscale connectome for the mouse, using enhanced green fluorescent protein-expressing adeno-associated viral vectors to trace axonal projections from defined regions and cell types, and high-throughput serial two-photon tomography to image the EGFP-labelled axons throughout the brain.
Spatiotemporal ontogeny of brain wiring
TLDR
It is demonstrated that a mechanism based on heterochronous and spatially ordered neurodevelopmental gradients, without the involvement of activity-dependent plasticity or axonal guidance cues, can reconstruct a large part of the wiring principles and global network topology of diverse adult brain connectomes, including fly and human connectomes.
Toward the Drosophila connectome: structural analysis of the brain network
TLDR
A first draft of the network architecture of the Drosophila connectome at the mesoscopic scale is proposed and two models for the flow of information associated with intuitive and reasoning behaviors, respectively are proposed.
Three-dimensional network of Drosophila brain hemisphere.
A Single-Cell Level and Connectome-Derived Computational Model of the Drosophila Brain
TLDR
The reconstructed Drosophila brain was structurally and dynamically more diverse than the randomized one and exhibited both Poisson-like and patterned firing activities and compared the reconstructed brain network with a randomized brain network by shuffling the connections of each neuron.
Combining genome-scale Drosophila 3D neuroanatomical data by bridging template brains
TLDR
This work constructs bridging registrations between template brains accounting for the vast majority of Drosophila melanogaster 3D neuroanatomical data and solves the related problem of mapping data between the left and right brain hemispheres via the construction of mirroring registrations.
Diverse Community Structures in the Neuronal-Level Connectome of the Drosophila Brain
TLDR
A neuron-to-neuron network of the Drosophila brain based on the 28,573 fluorescence images of single neurons in the newly released FlyCircuit v1.2 is constructed, and except for the two olfaction and the motor communities, the network is characterized by overall small-worldness.
A connectome and analysis of the adult Drosophila central brain
TLDR
Improved methods are summarized and the circuitry of a large fraction of the brain of the fruit fly Drosophila melanogaster is presented, reducing the effort needed to answer circuit questions, and procedures linking the neurons defined by the analysis with genetic reagents are provided.
...
...

References

SHOWING 1-10 OF 56 REFERENCES
A Proposal for a Coordinated Effort for the Determination of Brainwide Neuroanatomical Connectivity in Model Organisms at a Mesoscopic Scale
TLDR
A concerted effort is advocated for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brainwide coverage, using injections of tracers or viral vectors.
Mapping and manipulating neural circuits in the fly brain.
  • J. Simpson
  • Biology, Computer Science
    Advances in genetics
  • 2009
Genetic Dissection of Neural Circuits
Embryonic development of the Drosophila brain: formation of commissural and descending pathways.
TLDR
Analysis of axon pathway formation presented here reveals remarkable similarities as well as distinct differences in the embryonic development of the brain and the segmental ganglia, and forms the basis for a comprehensive genetic and molecular genetic dissection of axonal pathfinding processes in the developing brain.
Towards a virtual fly brain
TLDR
This work proposes to exploit the strengths of Drosophila central nervous system research to focus on a functional model that maps biologically plausible network architecture onto phenotypic data from neuronal inhibition and stimulation studies, leaving aside biophysical modelling of individual neuronal activity for future models until more data are available.
Clonal unit architecture of the adult fly brain.
TLDR
Construction of the brain by combining such groups of clonally related units would have been a simple and efficient strategy for building the complicated neural circuits during development as well as during evolution.
Cracking neural circuits in a tiny brain: new approaches for understanding the neural circuitry of Drosophila
...
...