Three‐yr follow‐up of a type 1 diabetes mellitus patient with an islet xenotransplant

  title={Three‐yr follow‐up of a type 1 diabetes mellitus patient with an islet xenotransplant},
  author={Rafael A Vald{\'e}s-Gonz{\'a}lez and David J. G. White and Luis M. Dorantes and Luis Ter{\'a}n and Guadalupe Nayely Garibay-Nieto and Eduardo Bracho-Blanchet and Roberto D{\'a}vila-P{\'e}rez and L Evia-Viscarra and Christopher E. Ormsby and Jorge-Tonatiuh Ayala-Sumuano and M.L. Silva‐Torres and Briceyda Ram{\'i}rez-Gonz{\'a}lez},
  journal={Clinical Transplantation},
Abstract:  In order to alleviate the shortage of human donors, the use of porcine islets of Langerhans for xenotransplantation in diabetic patients has been proposed as a solution. To overcome rejection, we have developed a procedure for protecting the islets by combining them with Sertoli cells and placing them in a novel subcutaneous device, that generates an autologous collagen covering. A type 1 diabetic woman was closely monitored for 10 months, and then transplanted in two devices with… 

Long‐term follow‐up of patients with type 1 diabetes transplanted with neonatal pig islets

These patients with type 1 diabetes transplanted with neonatal pig islets without immunosuppression achieved an excellent metabolic control after the first transplantation, which could explain the low frequency of chronic complications compared to patients with similar diabetes duration and age.

Correlation between insulin requirements and anti‐galactose antibodies in patients with type 1 diabetes transplanted with neonatal pig islets

The lowest anti‐Gal antibody levels were related to the highest insulin dose reductions, which could be explained by the device, Sertoli cells and accommodation process, and the lowest anti-pig antibody levels in xenotransplanted patients with type 1 diabetes and the relation to the clinical outcome.

Pig-to-Primate Islet Xenotransplantation: Past, Present, and Future

The results of pig-to-diabetic nonhuman primate islet xenotransplantation are steadily improving, with insulin independence being achieved for periods >1 year, and its potential for treatment of type 1 diabetes is focused on.

Islet cell transplantation for the treatment of type 1 diabetes in the USA.

The SUITO index is a simple and powerful tool to assess engrafted islet mass and is, therefore, useful for evaluating the efficacy of new immunosuppressant strategies once ICTx becomes a standard treatment, the donor shortage will become the next challenge.

Bioengineered Sites for Islet Cell Transplantation

The aim of this article is to review possible preclinical/clinical implantation sites for encapsulated islet transplantation as a function of the encapsulation design: macro/microcapsules and conformal coating.

Why do Japan's advanced medical treatments never get ahead?

The first Japanese islet cell transplant using non-heart beating donor in 2004 and the world's first successful case of living donor islet transplantation in 2005 were performed; however excellent glycemic control was able to be maintained for a prolonged period.

Clinical Allogeneic and Autologous Islet Cell Transplantation: Update

  • S. Matsumoto
  • Medicine, Biology
    Diabetes & metabolism journal
  • 2011
Autologous islet cell transplantation after total pancreatectomy for the treatment of chronic pancreatitis with severe abdominal pain is the standard therapy, even though only limited centers are able to perform this treatment.

Long-Term Metabolic Control of Autoimmune Diabetes in Spontaneously Diabetic Nonobese Diabetic Mice by Nonvascularized Microencapsulated Adult Porcine Islets

This is the first study to document long-term normalized HbA1c, porcine C peptide, and near normal glucose tolerance in immunosuppressed diabetic NOD mice transplanted intraperitoneally with microencapsulated API.

Cell-based treatments for diabetes.



Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: a 4-year study.

Long-term cell survival with concurrent positive effects on metabolic control are possible by this technique, and the presence of insulin-positive cells in the transplant was demonstrated by immunohistology in all 4 patients.

Islet Transplantation in Seven Patients with Type 1 Diabetes Mellitus Using a Glucocorticoid-Free Immunosuppressive Regimen

The observations in patients with type 1 diabetes indicate that islet transplantation can result in insulin independence with excellent metabolic control when glucocorticoid-free immunosuppression is combined with the infusion of an adequate islet mass.

Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol.

Islet transplantation has successfully corrected labile type 1 diabetes and problems with hypoglycemia, and the results show persistent insulin secretion, which is consistent with good glycemic control.

Immunoprotection of rat islet xenografts by cotransplantation with sertoli cells and a single injection of antilymphocyte serum1

Sertoli cells are able to prolong the survival of islet xenografts when combined with ALS, thereby supporting their use as a means to immunoprotect cellular grafts such as islets for the treatment of type 1 diabetes.

Long-term survival of nonhuman primates receiving life-supporting transgenic porcine kidney xenografts.

Normalization of renal function (urea and creatinine) in primate recipients of porcine renal xenografts suggests that pig kidneys may be suitable for future clinical xenotransplantation.

Cotransplantation of Allogeneic Islets With Allogeneic Testicular Cell Aggregates Allows Long-Term Graft Survival Without Systemic Immunosuppression

We prepared single-cell suspensions of Lewis rat (RT11/1) testicular cells and cultured these in vitro for 48 h under conditions that promoted the formation of cellular aggregates. In the absence of

Biological encapsulation as a new model for preservation of islets of Langerhans.

C-Peptide Determinations in Islet Xenotransplantation: A Study in the Pig-to-Mouse Model

In this study, injection of 10 times the physiological amount of porcine C-peptide into mice did not result in the excretion of the C- peptide in the urine, while after injection of radioactively labeled porcines into mice, the radioactive uptake in tissues belonging to the mononuclear phagocytic system was significantly increased in mice immunized towards the xenogeneic C-PEptide.

No Evidence of Infection with Porcine Endogenous Retrovirus in Recipients of Encapsulated Porcine Islet Xenografts

Screening of pigs from New Zealand herds for the presence and expression of the PERV was conducted, and it was established that different tissues of nude and nonobese diabetic mice previously transplanted with nonencapsulated pig islets were PERV DNA and RNA negative.

Testicular sertoli cells protect islet beta-cells from autoimmune destruction in NOD mice by a transforming growth factor-beta1-dependent mechanism.

It is concluded that Sertoli cell production of TGF-beta1, not FasL, protects islet beta-cells from autoimmune destruction andTGF- beta1 diverts islet-infiltrating cells from a beta-cell-destructive (IFN-gamma+) phenotype to a nondestructive (IL-4+) phenotype.