Three‐dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography

@article{Schrmann2018ThreedimensionalCS,
  title={Three‐dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography},
  author={Mirjam Sch{\"u}rmann and Gheorghe Cojoc and Salvatore Girardo and Elke Ulbricht and Jochen R. Guck and Paul M{\"u}ller},
  journal={Journal of Biophotonics},
  year={2018},
  volume={11}
}
Cells alter the path of light, a fact that leads to well‐known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI). ODT is complementary to fluorescence imaging and does not require any markers. The present study introduces RI and fluorescence tomography with optofluidic rotation (RAFTOR) of suspended cells, facilitating the segmentation of the 3D‐correlated RI and… 
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Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging
TLDR
An optical setup combining ODT with three-channel 3D fluorescence microscopy is presented, to enhance the molecular specificity of the 3D RI measurement.
Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D…
Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence
TLDR
The dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space unveiled interesting information inaccessible with previous methods, as demonstrated in the human immune cell (T cell) experiment.
Cell and nucleus refractive‐index mapping by interferometric phase microscopy and rapid confocal fluorescence microscopy
TLDR
Human colorectal adenocarcinoma cells are acquired and it is shown that the integral refractive index values are similar for the whole cell, the cytoplasm and the nucleus on the population level, but significantly different on the single cell level.
Computational approach to dark-field optical diffraction tomography
TLDR
Without changing the optical instruments used in ODT, subcellular organelles in live cells are clearly distinguished by applying a simple but effective computational approach that is validated by comparison with 3D epifluorescence images.
Limited-angle tomographic phase microscopy utilizing confocal scanning fluorescence microscopy.
We present a multimodal imaging technique, combining tomographic phase microscopy with limited angular projection range and number, and two-channel spinning-disk confocal scanning fluorescence…
Stabilization three-dimensional refractive-index reconstruction system of single suspension cell
TLDR
A combination strategy that uses acoustically oscillating bubble array based acoustofluidic device to simultaneously rotate cells in large-scale, and sets up a wDPM module to record cell quantitative phase maps with a high temporal and spatial phase sensitivity due to the common-path geometry and white light illumination is demonstrated.
Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy.
TLDR
A new multimodal imaging technique for quantitatively measuring the integral refractive index of the nuclei of live biological cells in suspension is suggested, obtaining nucleus refractiveIndex values that are lower than those of the cytoplasm, coinciding with recent findings.
Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy
TLDR
A new multimodal imaging technique for quantitatively measuring the integral refractive index of the nuclei of live biological cells in suspension is suggested, and it is believed that the proposed technique has the potential to be used for flow cytometry, where full 3-DRefractive index tomography is too slow to be implemented during flow.
Reconstructions of refractive index tomograms via a discrete algebraic reconstruction technique.
TLDR
This work presents an algorithm that accurately reconstructs RI tomography of a specimen with discrete and uniform RI, using prior information about the RI levels, and shows that it can precisely reconstruct RI tomograms of samples which have discrete and homogenous RI distributions in the presence of the missing information and noise.
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