Three‐dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography

  title={Three‐dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography},
  author={Mirjam Sch{\"u}rmann and Gheorghe Cojoc and Salvatore Girardo and Elke Ulbricht and Jochen R. Guck and Paul M{\"u}ller},
  journal={Journal of Biophotonics},
Cells alter the path of light, a fact that leads to well‐known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI). ODT is complementary to fluorescence imaging and does not require any markers. The present study introduces RI and fluorescence tomography with optofluidic rotation (RAFTOR) of suspended cells, facilitating the segmentation of the 3D‐correlated RI and… 
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A new multimodal imaging technique for quantitatively measuring the integral refractive index of the nuclei of live biological cells in suspension is suggested, and it is believed that the proposed technique has the potential to be used for flow cytometry, where full 3-DRefractive index tomography is too slow to be implemented during flow.
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