Three‐dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography

@article{Schrmann2018ThreedimensionalCS,
  title={Three‐dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography},
  author={Mirjam Sch{\"u}rmann and Gheorghe Cojoc and Salvatore Girardo and Elke Ulbricht and Jochen R. Guck and Paul M{\"u}ller},
  journal={Journal of Biophotonics},
  year={2018},
  volume={11}
}
Cells alter the path of light, a fact that leads to well‐known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI). ODT is complementary to fluorescence imaging and does not require any markers. The present study introduces RI and fluorescence tomography with optofluidic rotation (RAFTOR) of suspended cells, facilitating the segmentation of the 3D‐correlated RI and… 
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TLDR
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TLDR
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Reconstructions of refractive index tomograms via a discrete algebraic reconstruction technique.
TLDR
This work presents an algorithm that accurately reconstructs RI tomography of a specimen with discrete and uniform RI, using prior information about the RI levels, and shows that it can precisely reconstruct RI tomograms of samples which have discrete and homogenous RI distributions in the presence of the missing information and noise.
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