This Report Has Been Deumited and Cleared for Public Release under Dod Directive 5200,20 and No Restrictions ^re Imposed upon Its Use and Disclosure,

  • Vincent J. Ciccone
  • Published 2013

Abstract

A collagen-urease membrane complex of relatively stable enzymatic activity was successfully produced (and replicated numerous times) in our laboratory. In order to evaluate the potential use of this complex for the treatment of ur.incus wastewater, a biocatalytic reactor module of a novel spiral wound configuration was constructed and integrated into a prototype system containing reverse osmosis and ion exchange processes. The unit that was designed and constructed was sized so as to be capable of generating sufficient process data that could be used in the estimation of the system design parameters. These system design parameters could then be used to extrapolate the unit to other sizes for operation under larger feed flow rate conditions. This work was accomplished by using a synthetic urinous feed solution containing sodium chloride and urea as the major contaminants. The performance of the biocatalytic reactor module was simulated using a transport model consisting of factors which account for simultaneous mass transfer and biochemical reaction. The model was based upon the assumptions of steady-state, plug-flow, and Michaelis-Menten kinetics. From this model, the apparent Michaelis-Menten kinetic parameters were estimated using actual experimental data as the input Lo a direct search, parameter optimization scheme. These estimated parameters--Km = 0.0104 moles/liter

Cite this paper

@inproceedings{Ciccone2013ThisRH, title={This Report Has Been Deumited and Cleared for Public Release under Dod Directive 5200,20 and No Restrictions ^re Imposed upon Its Use and Disclosure,}, author={Vincent J. Ciccone}, year={2013} }