Thioredoxin reductase in human hepatoma cells is transcriptionally regulated by sulforaphane and other electrophiles via an antioxidant response element.

@article{Hintze2003ThioredoxinRI,
  title={Thioredoxin reductase in human hepatoma cells is transcriptionally regulated by sulforaphane and other electrophiles via an antioxidant response element.},
  author={Korry J. Hintze and Karl A Wald and Huawei Zeng and Elizabeth H. Jeffery and John W Finley},
  journal={The Journal of nutrition},
  year={2003},
  volume={133 9},
  pages={2721-7}
}
We previously reported the in vitro and in vivo induction of thioredoxin reductase (TR) by sulforaphane (SF) purified from broccoli. The present study was designed to determine whether this induction is mediated by putative antioxidant response elements (ARE) found in the promoter. Luciferase reporter constructs were built using the TR promoter sequence. Sulforaphane, tert-butylhydroquinone and beta-napthoflavone, as well as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA… CONTINUE READING