Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria.

Abstract

Mitochondria contain thioredoxin (Trx), a regulatory disulfide protein, and an associated flavoenzyme, NADP/Trx reductase, which provide a link to NADPH in the organelle. Unlike animal and yeast counterparts, the function of Trx in plant mitochondria is largely unknown. Accordingly, we have applied recently devised proteomic approaches to identify soluble Trx-linked proteins in mitochondria isolated from photosynthetic (pea and spinach leaves) and heterotrophic (potato tubers) sources. Application of the mitochondrial extracts to mutant Trx affinity columns in conjunction with proteomics led to the identification of 50 potential Trx-linked proteins functional in 12 processes: photorespiration, citric acid cycle and associated reactions, lipid metabolism, electron transport, ATP synthesis/transformation, membrane transport, translation, protein assembly/folding, nitrogen metabolism, sulfur metabolism, hormone synthesis, and stress-related reactions. Almost all of these targets were also identified by a fluorescent gel electrophoresis procedure in which reduction by Trx can be observed directly. In some cases, the processes targeted by Trx depended on the source of the mitochondria. The results support the view that Trx acts as a sensor and enables mitochondria to adjust key reactions in accord with prevailing redox state. These and earlier findings further suggest that, by sensing redox in chloroplasts and mitochondria, Trx enables the two organelles of photosynthetic tissues to communicate by means of a network of transportable metabolites such as dihydroxyacetone phosphate, malate, and glycolate. In this way, light absorbed and processed by means of chlorophyll can be perceived and function in regulating fundamental mitochondrial processes akin to its mode of action in chloroplasts.

3 Figures and Tables

0204060'04'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

485 Citations

Semantic Scholar estimates that this publication has 485 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Balmer2004ThioredoxinLR, title={Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria.}, author={Yves Balmer and William H. Vensel and Charlene K. Tanaka and William J. Hurkman and Eric Gelhaye and Nicolas Rouhier and Jean-Pierre Jacquot and Wanda Manieri and Peter Schuermann and Michel Droux and Bob B. Buchanan}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2004}, volume={101 8}, pages={2642-7} }