Thioredoxin is essential for Rhodobacter sphaeroides growth by aerobic and anaerobic respiration.

@article{Pasternak1997ThioredoxinIE,
  title={Thioredoxin is essential for Rhodobacter sphaeroides growth by aerobic and anaerobic respiration.},
  author={C{\'e}cile Pasternak and Karine Assemat and Jenny D. Clement-Metral and Gabriele Klug},
  journal={Microbiology},
  year={1997},
  volume={143 ( Pt 1)},
  pages={83-91}
}
To investigate the biological role of thioredoxin in the facultative photosynthetic bacterium Rhodobacter sphaeroides, attempts were made to construct a thioredoxin-deficient mutant by site-specific mutagenesis, using the Tn903 kanamycin resistance gene for selection. In situ and Southern hybridization analyses have demonstrated that the TrxA- mutation is lethal for R. sphaeroides growth under anaerobic conditions with DMSO as terminal electron acceptor and under aerobic conditions. In addition… CONTINUE READING