Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture.

@article{Koterba2012Thioredoxin1I,
  title={Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture.},
  author={Kristen L. Koterba and Trissa Borgschulte and Michael W Laird},
  journal={Journal of biotechnology},
  year={2012},
  volume={157 1},
  pages={261-7}
}
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We demonstrate a marked prevention of IgG1 disulfide bond reduction in a cell-density dependent manner by knocking down expression of TXN1 via lentivirus transduction of short hairpin RNA. 

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