Thionine-Modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications.

@article{Zasoska2015ThionineModifiedPM,
  title={Thionine-Modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications.},
  author={Beata Anna Zasońska and Michaela {\vC}adkov{\'a} and Aneta Kov{\'a}řov{\'a} and Zuzana B{\'i}lkov{\'a} and Lucie Koreck{\'a} and Daniel Hor{\'a}k},
  journal={ACS applied materials & interfaces},
  year={2015},
  volume={7 44},
  pages={24926-31}
}
Monodisperse poly(glycidyl methacrylate) (PGMA) nanospheres were obtained by emulsifier-free emulsion polymerization and characterized by physicochemical methods. The effects of various reaction parameters on the particle properties were investigated. The particle size was controlled in the range of 350-420 nm. To introduce carboxyl groups, the PGMA nanospheres were hydrolyzed and oxidized with KMnO4. Subsequently, the enzyme horseradish peroxidase (HRP) and the electron mediator thionine were… CONTINUE READING