Thiol-maleimide poly(ethylene glycol) crosslinking of L-asparaginase subunits at recombinant cysteine residues introduced by mutagenesis

@inproceedings{RamirezPaz2018ThiolmaleimidePG,
  title={Thiol-maleimide poly(ethylene glycol) crosslinking of L-asparaginase subunits at recombinant cysteine residues introduced by mutagenesis},
  author={Josell Ramirez-Paz and Manoj Saxena and Louis J Delinois and Freisa M Joaqu{\'i}n-Ovalle and Shiru Lin and Zhongfang Chen and Virginia A Rojas-Nieves and Kai Griebenow},
  booktitle={PloS one},
  year={2018}
}
L-Asparaginase is an enzyme successfully being used in the treatment of acute lymphoblastic leukemia, acute myeloid leukemia, and non-Hodgkin's lymphoma. However, some disadvantages still limit its full application potential, e.g., allergic reactions, pancreatitis, and blood clotting impairment. Therefore, much effort has been directed at improving its… CONTINUE READING