Thiol‐reactive dyes for fluorescence labeling of proteomic samples

@article{Tyagarajan2003ThiolreactiveDF,
  title={Thiol‐reactive dyes for fluorescence labeling of proteomic samples},
  author={K. Tyagarajan and E. Pretzer and J. Wiktorowicz},
  journal={ELECTROPHORESIS},
  year={2003},
  volume={24}
}
Covalent derivatization of proteins with fluorescent dyes prior to separation is increasingly used in proteomic research. This paper examines the properties of several commercially available iodoacetamide and maleimide dyes and discusses the conditions and caveats for their use in labeling of proteomic samples. The iodoacetamide dyes BODIPY TMR cadaverine IA and BODIPY Fl C1‐IA were highly specific for cysteine residues and showed little or no nonspecific labeling even at very high dye:thiol… Expand
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