Thioglucose-stabilized gold nanoparticles as a novel platform for colorimetric bioassay based on nanoparticle aggregation.

@article{Watanabe2010ThioglucosestabilizedGN,
  title={Thioglucose-stabilized gold nanoparticles as a novel platform for colorimetric bioassay based on nanoparticle aggregation.},
  author={Shigeru Watanabe and Kazuma Yoshida and Keitarou Shinkawa and Daisuke Kumagawa and Hideki Seguchi},
  journal={Colloids and surfaces. B, Biointerfaces},
  year={2010},
  volume={81 2},
  pages={570-7}
}
Gold nanoparticles stabilized with thioglucose (TGlu-AuNPs), which have carboxyl groups on the particle surface as anchoring sites for covalent immobilization of biomolecules, were prepared by the chemical reduction of HAuCl4 using 1-thio-β-D-glucose as a reducing and stabilizing agent, and their application to colorimetric bioassay was demonstrated using the carbohydrate-lectin system. p-Aminophenyl α-D-mannose (Man-NH2) was covalently attached by a conventional method to the activated… CONTINUE READING