Corpus ID: 89520289

Thiobenzamide S-oxidation in Perfused Rat Liver: Ex Vivo Determination of Hepatic Flavin-Containing Monooxygenase Activity

@article{Chung1997ThiobenzamideSI,
  title={Thiobenzamide S-oxidation in Perfused Rat Liver: Ex Vivo Determination of Hepatic Flavin-Containing Monooxygenase Activity},
  author={W. Chung and H. Roh and Y. Cha},
  journal={The Korean Journal of Physiology and Pharmacology},
  year={1997},
  volume={1},
  pages={591-595}
}
An ex vivo assay determining the flavin-containing monooxygenase (FMO) activity in perfused rat liver has been developed by assessing the rate of thiobenzamide S-oxide (TBSO) formation from the infused thiobenzamide (TB). The hepatotoxicity by TB or TBSO was not a critical factor for maintaining the FMO activity for up to 50 min. The FMO activity expressed in nmoles TBSO produced/g liver/min was the same for the recycling and non-recycling perfusion. This implies that reduction of the oxidized… Expand