Thin-layer chromatography.

@article{Meyers2008ThinlayerC,
  title={Thin-layer chromatography.},
  author={Caren L. Freel Meyers and David J. Meyers},
  journal={Current protocols in nucleic acid chemistry},
  year={2008},
  volume={Appendix},
  pages={
          3D
        }
}
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (R(f)). Candidate compounds can be co-spotted… CONTINUE READING

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