Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.)

@article{Mithila2002ThidiazuronIS,
  title={Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.)},
  author={Jugulam Mithila and J. Christopher Hall and J. M. R. Victor and Praveen K. Saxena},
  journal={Plant Cell Reports},
  year={2002},
  volume={21},
  pages={408-414}
}
Abstract. Regeneration via shoot organogenesis and somatic embryogenesis was observed from thidiazuron (TDZ)-treated leaf and petiole explants of greenhouse- and in vitro-grown African violet plants. The response of cultures to other growth regulators over a range of 0.5 µM to 10 µM was 50% less than that observed with TDZ. A comparative study among several cultivars of African violet indicated that 'Benjamin' and 'William' had the highest regeneration potential. In 'Benjamin', higher… 
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References

SHOWING 1-10 OF 26 REFERENCES
Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves
Thidiazuron (TDZ), a substituted urea with cytokinin activity, was significantly more effective than benzyladenine (BA) at inducing organogenesis from detached Rubus cotyledons and leaves. The
In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture
TLDR
A micropropagation system was developed for E. purpurea that will provide sterile plant material for further investigations into medicinally active biochemicals and may facilitate mass production of high-quality E. Purpurea plants for the commercial market.
Thidiazuron‐induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons
TLDR
It appeared that TDZ induced somatic embryogenesis in peanut by influencing endogenous levels of both auxin and cytokinins, while TDZ itself was detected only in the cotyledons.
Morphoregulatory role of thidiazuron : substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures.
Somatic embryogenesis was induced in hypocotyl explants of geranium (Pelargonium x hortorum) cultured on media supplemented with various concentrations of N-phenyl-N'-1,2,3-thiadiazol-5-ylurea
In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron
TLDR
Histological investigations confirmed the switch in the regeneration pathway from directly formed adventitious shoots to embryogenesis and TDZ was found to be more effective compared to BAP as an inductive signal of regeneration.
Thidiazuron induces high-frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris)
TLDR
Of various cytokinins or compounds with cytokinin-like activity tested for inducing shoot formation in pea seeds cultures,TDZ was found to be most effective and inductive capability of TDZ was then tested in several other genotypes of Pisum sativum and two other large-seeded grain legumes.
Rapid adventitious shoot regeneration from leaf explants of European birch
TLDR
This reliable shoot regeneration system can be used for rapid shoot proliferation and potentially for genetic engineering of European birch.
Cytokinins, donor plants and time in culture affect shoot regenerative capacity of American elm leaves
TLDR
Explants from productive donor plants should be placed on DKW medium supplemented with TDZ to improve shoot regeneration efficiency from American elm leaves.
The role of proline in thidiazuron-induced somatic embryogenesis of peanut
TLDR
The results indicate that proline plays a key role in directing the route of TDZ-induced somatic embryogenesis and that TDZ effectively stimulates a cascade of metabolic events resulting in the production of specific metabolites, including amino acids, required for the regenerative process.
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