Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.)
@article{Mithila2002ThidiazuronIS, title={Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.)}, author={Jugulam Mithila and J. Christopher Hall and J. M. R. Victor and Praveen K. Saxena}, journal={Plant Cell Reports}, year={2002}, volume={21}, pages={408-414} }
Abstract. Regeneration via shoot organogenesis and somatic embryogenesis was observed from thidiazuron (TDZ)-treated leaf and petiole explants of greenhouse- and in vitro-grown African violet plants. The response of cultures to other growth regulators over a range of 0.5 µM to 10 µM was 50% less than that observed with TDZ. A comparative study among several cultivars of African violet indicated that 'Benjamin' and 'William' had the highest regeneration potential. In 'Benjamin', higher…
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An efficient and simple procedure for inducing high frequency direct shoot organogenesis and somatic embryogenesis in lentil from cotyledonary node explants in response to TDZ alone is reported and the possible role of stress in thidiazuron induced somatic embryos is discussed.
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- Biology, MedicineScientific reports
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Somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology, and this protocol provides a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species.
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