Thidiazuron-Induced High-Frequency Shoot Regeneration from Root Region of Robinia pseudoacacia L. Seedlings

  title={Thidiazuron-Induced High-Frequency Shoot Regeneration from Root Region of Robinia pseudoacacia L. Seedlings},
  author={Mahdi Hosseini-Nasr and Abdur Rashid},
  journal={Biologia Plantarum},
High-frequency regeneration of shoots was achieved at root region of seedlings of Robinia pseudoacacia L. cultured from seeds on medium supplemented with thidiazuron (TDZ, 1.0 μM). The roots of intact seedlings proliferated and formed a compact callus followed by differentiation of numerous shoots. Corresponding cultures on benzylaminopurine-containing medium exhibited much weaker response. Hypocotyl segments also formed shoots at a lower concentration of TDZ (0.1 μM). The shoots formed on TDZ… CONTINUE READING

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