Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida

@article{Thirukkumaran2009ThidiazuronAE,
  title={Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida},
  author={Gunaratnam Thirukkumaran and Valentine Otang Ntui and Raham Sher Khan and Masahiro Mii},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2009},
  volume={99},
  pages={109-115}
}
Efficient shoot regeneration and Agrobacterium-mediated genetic transformation systems were developed for Petunia hybrida cv. Mitchell. Leaf explants of petunia were cultured on Murashige and Skoog (MS) medium with different concentrations of thidiazuron (TDZ) without auxin. The highest frequency of shoot regeneration (52.1%) and mean number of shoots per explant (4.1) were obtained on medium containing 2 mg l−1 TDZ. Leaf explants inoculated with Agrobacterium tumefaciens strain EHA101/pIG121Hm… CONTINUE READING

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