Biochemical properties of Hsp70 chaperone system from Meiothermus ruber
- Biology, Chemistry
It is demonstrated that, in contrast to Thermus thermophilus, both M.Hsp40 and Mru.
Diversity in transcripts and translational pattern of stress proteins in marine extremophiles
In vitro studies conducted on the transcripts and translational pattern of stress proteins in extremophiles highlight that among all the stressful conditions, salt/osmotic stress evokes the expression of highest number of transcripts/proteins while psychrophilic condition the least.
Proton and cadmium adsorption by the archaeon Thermococcus zilligii: Generalising the contrast between thermophiles and mesophiles as sorbents
- Chemistry, Engineering
Kinetics of the electron transfer reaction of Cytochrome c552 adsorbed on biomimetic electrode studied by time-resolved surface-enhanced resonance Raman spectroscopy and electrochemistry
- ChemistryEuropean Biophysics Journal
The present work focuses on the kinetic part of the heterogeneous ET process of Cyt-c552 adsorbed onto electrodes coated with mixed SAMs of different alkyl chain length, and shows that the kinetic constant values obtained by TR-SERR spectroscopy could be compared to those obtained from cyclic voltammetry.
A programmable pAgo nuclease with RNA target preference from the psychrotolerant bacteria Mucilaginibacter paludis
It is demonstrated that MbpAgo is a unique programmable nuclease that has a strong preference for RNA targets, with great potential applications in the field of nucleic acid biotechnology.
A programmable pAgo nuclease with universal guide and target specificity from the mesophilic bacterium Kurthia massiliensis
A unique KmAgo nuclease from the mesophilic bacterium Kurthia massiliensis that can be programmed with either DNA or RNA guides and can precisely cleave both DNA and RNA targets is described.
A short prokaryotic argonaute cooperates with membrane effector to confer antiviral defense
It is revealed that a short pAgo protein in Sulfolobus islandicus, together with its two genetically associated proteins, Aga1 and Aga2, provide robust antiviral protection via abortive infection and highlight the cooperation between pAgos and their widespreadassociated proteins, suggesting an uncharted diversity of pAGO-derived immune systems that await to be discovered.
The Clustered Regularly Interspaced Short Palindromic Repeat System and Argonaute: An Emerging Bacterial Immunity System for Defense Against Natural Transformation?
- BiologyFrontiers in Microbiology
The functions and mechanisms of the CRISPR system and Agos in cellular defense against natural transformation are discussed and how antagonization of these systems occurs during natural transformation is discussed.
Pull in and Push Out: Mechanisms of Horizontal Gene Transfer in Bacteria
- BiologyFront. Microbiol.
The discovery of cell-to-cell contact-dependent plasmid transformation implies the presence of additional mechanism(s) of transformation, which complementing the existing methods of DNA transfer among bacteria.
Survival and Energy Producing Strategies of Alkane Degraders Under Extreme Conditions and Their Biotechnological Potential
- Biology, EngineeringFront. Microbiol.
Many petroleum-polluted areas are considered as extreme environments because of co-occurrence of low and high temperatures, high salt, and acidic and anaerobic conditions. Alkanes, which are major…