Thermus aquaticus DNA polymerase I mutants with altered fidelity. Interacting mutations in the O-helix.

@article{Suzuki2000ThermusAD,
  title={Thermus aquaticus DNA polymerase I mutants with altered fidelity. Interacting mutations in the O-helix.},
  author={Motoshi Suzuki and Shonen Yoshida and E. Adman and Annika Blank and L. A. Loeb},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 42},
  pages={32728-35}
}
Phe(667) in the conserved O-helix of Thermus aquaticus (Taq) DNA polymerase I (pol I) is known to be important for discrimination against dideoxy-NTPs. We show here that Phe(667) is also important for base selection fidelity. In a forward mutation assay at high polymerase concentration, wild type pol I catalyzed frequent A --> T and G --> T transversions and -1 frameshifts at nonreiterated sites involving loss of a purine immediately downstream of a pyrimidine. The mutants F667L and A661E,I665T… CONTINUE READING

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