Thermostable beta-galactosidase from an extreme thermophile, Thermus sp. A4: enzyme purification and characterization, and gene cloning and sequencing.

@article{Ohtsu1998ThermostableBF,
  title={Thermostable beta-galactosidase from an extreme thermophile, Thermus sp. A4: enzyme purification and characterization, and gene cloning and sequencing.},
  author={Naomi Ohtsu and Hidemasa Motoshima and Kazunori Goto and Fuji Tsukasaki and Hiroshi Matsuzawa},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1998},
  volume={62 8},
  pages={1539-45}
}
We purified and characterized a thermophilic beta-galactosidase from Thermus sp. A4 isolated from the Atagawa hot spring (Shizuoka, Japan). The enzyme was monomeric, and its molecular mass was estimated to be 75 kDa by SDS-polyacrylamide gel electrophoresis. The enzyme was extremely thermostable and retained its full activity after incubation at 70 degrees C for 20 h. The Km observed were 5.9 mM for ortho-nitrophenyl beta-D-galactopyranoside and 19 mM for lactose. We cloned and analyzed the… CONTINUE READING
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