Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution.

@article{Bakke2006ThermostabilizationOP,
  title={Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution.},
  author={Mikio Bakke and Chiaki Setoyama and Retsu Miura and Naoki Kajiyama},
  journal={Biotechnology and bioengineering},
  year={2006},
  volume={93 5},
  pages={1023-7}
}
D-amino acid oxidase (DAO) is of considerable practical importance, such as bioconversion and enzymatic assay. In this study, we succeeded in obtaining a thermostable mutant DAO from porcine kidney by a single amino acid substitution. This mutant enzyme, F42C, was stable at 55 degrees C, while the wild-type enzyme was stable only up to 45 degrees C. The Km values of F42C for D-amino acids was about half of those of the wild-type enzyme. This mutant DAO with improved stability and affinity for… CONTINUE READING