Thermostabilization of extremophilic Dictyoglomus thermophilum GH11 xylanase by an N-terminal disulfide bridge and the effect of ionic liquid [emim]OAc on the enzymatic performance.

@article{Li2013ThermostabilizationOE,
  title={Thermostabilization of extremophilic Dictyoglomus thermophilum GH11 xylanase by an N-terminal disulfide bridge and the effect of ionic liquid [emim]OAc on the enzymatic performance.},
  author={He Li and Anna Kankaanp{\"a}{\"a} and Hairong Xiong and Michael Hummel and Herbert Sixta and Heikki Ojamo and Ossi Turunen},
  journal={Enzyme and microbial technology},
  year={2013},
  volume={53 6-7},
  pages={414-9}
}
In the present study, an extremophilic GH11 xylanase was stabilized by an engineered N-terminal disulphide bridge. The effect of the stabilization was then tested against high temperatures and in the presence of a biomass-dissolving ionic liquid, 1-ethyl-3-methylimidazolium acetate ([emim]OAc). The N-terminal disulfide bridge increased the half-life of a GH11 xylanase (XYNB) from the hyperthermophilic bacterium Dictyoglomus thermophilum by 10-fold at 100°C. The apparent temperature optimum… CONTINUE READING
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