Thermoresponsive poly(glycidyl ether) brushes on gold: Surface engineering parameters and their implication for cell sheet fabrication.

@article{Heinen2017ThermoresponsivePE,
  title={Thermoresponsive poly(glycidyl ether) brushes on gold: Surface engineering parameters and their implication for cell sheet fabrication.},
  author={Silke Heinen and Jos{\'e} Luis Cu{\'e}llar-Camacho and Marie Weinhart},
  journal={Acta biomaterialia},
  year={2017},
  volume={59},
  pages={
          117-128
        }
}
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19 Citations
Background Citations
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Methods Citations
1
Results Citations
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19 Citations

Transfer of functional thermoresponsive poly(glycidyl ether) coatings for cell sheet fabrication from gold to glass surfaces.
TLDR
Self-assembled monolayer brushes of PGE-AA block copolymers on glass are functional and stable thermoresponsive coatings for application in cell sheet fabrication of human fibroblasts as determined by X-ray photoelectron spectroscopy.
Glycidyl Ether-Based Coatings on Polystyrene Culture Substrates for Temperature-Triggered Cell Sheet Fabrication
In this study, we introduce a platform to fabricate human dermal fibroblast (HDF), human aortic smooth muscle cell (HAoSMC) and human umbilical vein endothelial cell (HUVEC) sheets using…
Switchable Oligo(glycidyl ether) Acrylate Bottlebrushes "Grafted-from" Polystyrene Surfaces: A Versatile Strategy toward Functional Cell Culture Substrates.
TLDR
This study demonstrates the potential of OGEA-based coatings for cell sheet fabrication by adjusting the bottlebrush properties via the grafting procedure and coating structure and was able to harvest confluent HDF sheets from functionalized PS substrates in a temperature-triggered, controlled manner.
Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition
TLDR
It is shown that the temperature-dependent switchability of the brush coatings is not only dependent on the cloud point temperature of the block copolymers, but also markedly governed by the hydrophobicity of the surface-bound benzophenone anchor and the subjacent substrate material.
Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting
TLDR
Temperature dependent adhesive properties of poly(N-isopropylacrylamide) (PNIPAm) microgel coated surfaces (PMS) are characterized using various atomic force microscopy based approaches to gain a deeper understanding of cell-sheet maturation and harvesting process and the involved partial ECM dissolution.
Endothelial, smooth muscle and fibroblast cell sheet fabrication from self-assembled thermoresponsive poly(glycidyl ether) brushes.
TLDR
It is found that, by decreasing the temperature from 37 to 20 °C, the coatings undergo a pancake-to-brush transition, which triggers cell sheet detachment, which can be applied in vascular tissue engineering.
Cruciate Ligament Cell Sheets Can Be Rapidly Produced on Thermoresponsive poly(glycidyl ether) Coating and Successfully Used for Colonization of Embroidered Scaffolds
TLDR
Anterior cruciate ligament (ACL) cell sheets combined with biomechanically competent scaffolds might facilitate ACL tissue engineering and PGE coating facilitates ligamentocyte sheet formation, and sheets colonizing the scaffolds displayed a ligament-related phenotype.
Thermoresponsive Poly(glycidyl ether)s for Cell Sheet Fabrication
1 INTRODUCTION 1 1.1 General Aspects of Cell Sheet Engineering 1 1.2 Thermoresponsive Polymers 3 1.2.1 Phase Transition 3 1.2.2 Determination of Phase Transition Temperatures in Solution 5 1.2.3…
On the Foundation of Thermal "Switching": The Culture Substrate Governs the Phase Transition Mechanism of Thermoresponsive Brushes and their Performance in Cell Sheet Fabrication.
Transiently thermally responsive surfaces: Concepts for cell sheet engineering
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