Thermophoresis of single stranded DNA

@article{Reineck2010ThermophoresisOS,
  title={Thermophoresis of single stranded DNA},
  author={Philipp Reineck and Christoph J. Wienken and Dieter Braun},
  journal={ELECTROPHORESIS},
  year={2010},
  volume={31}
}
The manipulation and analysis of biomolecules in native bulk solution is highly desired; however, few methods are available. In thermophoresis, the thermal analog to electrophoresis, molecules are moved along a microscopic temperature gradient. Its theoretical foundation is still under debate, but practical applications for analytics in biology show considerable potential. Here we measured the thermophoresis of highly diluted single stranded DNA using an all‐optical capillary approach… Expand
Biotechnological applications of thermophoresis
For over 150 years it is known that particles in a temperature gradient conduct a directed movement. This is called the Soret effect or thermophoresis. Still the underlying physical principles ofExpand
Thermophoretic melting curves quantify the conformation and stability of RNA and DNA
TLDR
The thermophoretic analysis of nucleic acids shows the technique’s versatility for the investigation ofucleic acids relevant in cellular processes like RNA interference or gene silencing. Expand
Thermophoretic manipulation of DNA translocation through nanopores.
TLDR
The theoretical evaluation shows that the DNA translocation speeds will be orders smaller than the electrophoretic counterpart, while high capture rate of DNA into nanopore is maintained, both of which would greatly benefit the sequencing. Expand
Thermophoresis for characterizing biomolecular interaction.
TLDR
In this review, microscale thermophoresis technology (MST) is presented as an analytical technique for characterizing biomolecular interactions and is found to be a powerful technique in quantitation of binding events based on the movement of molecules in microscopic temperature gradient. Expand
Concentration-dependent thermophoretic accumulation for the detection of DNA using DNA-functionalized nanoparticles.
TLDR
The thermophoresis-based biosensing method is found to be simple and effective for detecting DNA and the hybridization between the specially designed capture probes and the target DNA significantly changes the thermophoretic properties of the fluorescent probes. Expand
Ionic thermophoresis and its application in living cells
Although thermophoresis, i.e. the directed movement of molecules in a temperature gradient, was discovered more than 150 years ago, its molecular origin is not jet fully understood. NonethelessExpand
Linearisation of λDNA molecules by instantaneous variation of the trapping electrode voltage inside a micro-channel
Because long DNA molecules usually exist in random coil states due to the entropic effect, linearisation is required for devices equipped with nanopores where electrical sequencing is necessaryExpand
Escalation of polymerization in a thermal gradient
TLDR
The results confirm that a thermal gradient can significantly enlarge the available sequence space for the emergence of catalytically activePolymerization and accumulation become mutually self-enhancing and result in a hyperexponential escalation of polymer length. Expand
Optical fluid and biomolecule transport with thermal fields.
TLDR
From the short but intense history of applying thermal fields to control fluid flow and biological molecules, it is inferred that many unexpected and highly synergistic effects and applications are likely to be explored in the future. Expand
Optical thermophoresis for quantifying the buffer dependence of aptamer binding.
TLDR
A new immobilization-free method in which thermophoresis, the movement of molecules in a thermal gradient, is used to determine binding curves, which can be used to study biomolecular binding reactions in various buffers as well as in human blood serum. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 33 REFERENCES
Trapping of DNA by thermophoretic depletion and convection.
TLDR
The thermal diffusion constant D(T)=0.4x10(-8) cm(2)/s K for DNA is quantified for the first time and offers a new approach to biological microfluidics and replicating systems in prebiotic evolution. Expand
Why molecules move along a temperature gradient
  • S. Duhr, D. Braun
  • Medicine, Chemistry
  • Proceedings of the National Academy of Sciences
  • 2006
TLDR
Using an all-optical microfluidic fluorescence method, results are presented that determine the effective charge of DNA and beads over a size range that is not accessible with electrophoresis, and support a unifying theory based on solvation entropy. Expand
Charging behavior of single-stranded DNA polyelectrolyte brushes.
TLDR
The data indicate a reorganization of the monolayer with changes in ionic strength and strand coverage that is consistent with that expected for a polyelectrolyte brush, and a description of monolayers behavior within a polymer science framework is formulated. Expand
The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides.
  • G. S. Manning
  • Chemistry, Medicine
  • Quarterly reviews of biophysics
  • 1978
TLDR
An analogous situation existed in the field of protein chemistry during the period after the formulation and confirmation of the Debye—Huckel theory of ionic solutions but before Scatchard's incorporation of the theory into his analysis of the binding properties of proteins. Expand
Diffusion coefficient of DNA molecules during free solution electrophoresis
TLDR
An experimental study of the diffusion coefficient of both ssDNA and dsDNA molecules during free‐flow electrophoresis shows that a simplistic use of Nernst‐Einstein's relation fails, and that the electric field actually has no effect on the thermal diffusion process. Expand
Persistence Length of Single-Stranded DNA
The self-diffusion coefficient of a series of DNA fragments ranging from 280 to 5386 bases has been measured by fluorescence recovery after photobleaching after thermal denaturation in 8 M urea. TheExpand
Thermophoresis in protein solutions
Thermophoresis, unlike thermal diffusion in simple mixtures, consists in particle drift induced by a temperature gradient ∇T. We show that thermophoresis in lysozyme solutions has a very distinctiveExpand
Melting curve analysis in a snapshot
The thermal denaturation of molecules is an essential method in biochemistry and diagnostics, including the measurement of single nucleotide polymorphisms and the binding analysis of proteins. WeExpand
Optothermal molecule trapping by opposing fluid flow with thermophoretic drift.
TLDR
This work superpose fluid flow with thermophoretic molecule flow under well-defined microfluidic conditions and shows how a radially converging temperature field confines short DNA into a 10 microm small spot. Expand
Does thermophoretic mobility depend on particle size?
TLDR
The thermophoretic mobility (or "thermal diffusion coefficient") D(T) is basically independent on particle size, in particular, when the interfacial properties of the colloidal particles are carefully standardized by adsorbing a surfactant layer on the particle surface. Expand
...
1
2
3
4
...