Thermophilic bacteria in cool temperate soils: are they metabolically active or continually added by global atmospheric transport?

  title={Thermophilic bacteria in cool temperate soils: are they metabolically active or continually added by global atmospheric transport?},
  author={Roger Marchant and Andrea Franzetti and Spyros G. Pavlostathis and Didem Okutman Taş and Isabel Erdbrűgger and Ali Űnyayar and Mehmet Ali Mazmancı and Ibrahim M. Banat},
  journal={Applied Microbiology and Biotechnology},
Thermophilic soil geobacilli isolated from cool temperate geographical zone environments have been shown to be metabolically inactive under aerobic conditions at ambient temperatures (−5 to 25°C). It is now confirmed that a similar situation exists for their anaerobic denitrification activity. It is necessary therefore to determine the mechanisms that sustain the observed significant viable populations in these soils. Population analysis of thermophiles in rainwater and air samples has shown… 

Are thermophilic microorganisms active in cold environments?

Abstract The mean air temperature of the Icelandic interior is below 10 °C. However, we have previously observed 16S rDNA sequences associated with thermophilic lineages in Icelandic basalts.

Presence and potential role of thermophilic bacteria in temperate terrestrial environments

Quantitative, real-time reverse-transcription polymerase chain reaction on environmental samples indicated that the examined thermophilic Firmicutes represented up to 3.4% of the total bacterial community RNA.

The Geobacillus paradox: why is a thermophilic bacterial genus so prevalent on a mesophilic planet?

Examination of Geobacilli genome sequences reveals that they are endowed with a battery of sensors, transporters and enzymes dedicated to hydrolysing plant polysaccharides, enabling them to be mobilized in the atmosphere and transported long distances.

Latitude-dependent underestimation of microbial extracellular enzyme activity in soils

Decomposition of soil organic matter by microorganisms is a major process governing the carbon balance between soil and atmosphere which needs to be fully understood. Extracellular enzyme activity is

“Freezing” Thermophiles: From One Temperature Extreme to Another

New detections of thermophiles in psychrobiotic (i.e., bearing cold-tolerant life forms) marine and terrestrial habitats including Arctic marine sediments, Antarctic accretion ice, permafrost, and

Putative Nickel-Dependent Anaerobic Carbon Monoxide Uptake Occurs Commonly in Soils and Sediments at Ambient Temperature and Might Contribute to Atmospheric and Sub-Atmospheric Carbon Monoxide Uptake During Anoxic Conditions

Carbon monoxide (CO) occurs naturally in the atmosphere where it plays a critical role in tropospheric chemistry. Atmospheric CO uptake by soils has been well documented as an important CO sink and

Freezing Tolerance of Thermophilic Bacterial Endospores in Marine Sediments

Results show that freezing impacts but does not obliterate thermospore populations and their ability to germinate and grow under appropriate conditions, important for assessing ther atmospore viability in frozen samples and following cold exposure such as the very low temperatures that would be encountered during panspermia.

Cultivation With Powdered Meteorite (NWA 1172) as the Substrate Enhances Low-Temperature Preservation of the Extreme Thermoacidophile Metallosphaera sedula

Recent studies have uncovered a vast number of thermophilic species in icy environments, permanently cold ocean sediments, cold sea waters, and cool soils. The survival of thermophiles in

Life in High-Temperature Environments

A significant recent advancement of the study of life at high temperature is the use of single-cell genomics and metagenomics approaches to probe yet-uncultivated lineages in high-temperature habitats; however, this progress must be matched with an equally vigorous program to test functions predicted from these genomes.

An “overlooked” habitat for thermophilic bacteria: the phyllosphere

The hypothesis that the phyllosphere (surface of leaves), due to its exposition to the sun, could well be a thermophilic habitat for microbes and thus a source of thermophobic microbes growing around 50°C – 60°C is explored and a life cycle of microbes belonging to the thermophile biotope associated with leaf surfaces is proposed.



The frequency and characteristics of highly thermophilic bacteria in cool soil environments.

Biochemical testing of five strains reveals a significant ability to utilize alkanes and some aromatic hydrocarbons and the question is raised of how these organisms, which are apparently unable to grow at the temperatures experienced in these cool soils, are so prominent.

The degradation of n-hexadecane in soil by thermophilic geobacilli.

A fragment of the alkane mono-oxygenase gene (alkB) was amplified from thermophilic Geobacillus thermoleovorans strain T70 by PCR using degenerate primers to determine if expression of the gene was inducible in the presence of an alkane or constitutively expressed in soil.

High growth rate and substrate exhaustion results in rapid cell death and lysis in the thermophilic bacterium Geobacillus thermoleovorans

Batch cultures of the thermophilic bacterium Geobacillus thermoleovorans T80 attained extremely high‐specific glucose utilization rates leading to high specific growth rates, followed by extensive cell death and lysis with the onset of substrate exhaustion, a lytic phenomenon observed when a range of different carbon substrates reached near exhaustion.

Phylogeny of Microorganisms Populating a Thick, Subaerial, Predominantly Lithotrophic Biofilm at an Extreme Acid Mine Drainage Site

Microenvironments of low redox potential possibly exist in the predominantly oxidizing environments of the slime, and this results expand the knowledge of the biodiversity of acid mine drainage environments and extend the understanding of the ecology of extremely acidic systems.

Isolation ofBacillus schlegelii, a thermophilic, hydrogen oxidizing, aerobic autotroph, from geothermal and nongeothermal environments

Phenotypic similarities, as well as DNA G+C content and DNA:DNA homologies, clearly revealed that the isolated strains belonged to the taxospecies B. schlegelii, and strains found in cold environments are probably allochthonous, their origin being geothermal and volcanic areas.

Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.

The physiological, biochemical and DNA-DNA hybridization studies of strain Ga(T) showed that it differs both genotypically and phenotypically from the recognized Geobacillus species.

Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillu

Comparative analysis of the 16S rDNA sequences and fatty acid compositions of the novel isolates and established species of thermophilic bacilli indicated that the subsurface strains represent two new species within a new genus, for which the names Geobacillus subterraneus gen. nov., sp.nov.

Upper boundary of the biosphere

The presence of pigmented microbial forms leads us to believe that natural selection is occurring in the mesosphere because cells possessing chromogenous pigments (carotenoids, melanins) are more resistant to ultraviolet-ray action.