Thermodynamics of single peptide bond cleavage in bovine pancreatic trypsin inhibitor (BPTI).

Abstract

A major goal of this paper was to estimate a dynamic range of equilibrium constant for the opening of a single peptide bond in a model protein, bovine pancreatic trypsin inhibitor (BPTI). Ten mutants of BPTI containing a single Xaa-->Met substitution introduced in different parts of the molecule were expressed in Escherichia coli. The mutants were folded… (More)

Topics

  • Presentations referencing similar topics