Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1.

@article{Revers2002ThermoconditionalMO,
  title={Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1.},
  author={Lu{\'i}s Fernando Revers and Jacqueline Moraes Cardone and Diego Bonatto and Jenifer Saffi and Martin Grey and Heinz Feldmann and Martin Brendel and Jo{\~a}o Antonio P{\^e}gas Henriques},
  journal={Nucleic acids research},
  year={2002},
  volume={30 22},
  pages={4993-5003}
}
The conditionally-lethal pso4-1 mutant allele of the spliceosomal-associated PRP19 gene allowed us to study this gene's influence on pre-mRNA processing, DNA repair and sporulation. Phenotypes related to intron-containing genes were correlated to temperature. Splicing reporter systems and RT-PCR showed splicing efficiency in pso4-1 to be inversely correlated to growth temperature. A single amino acid substitution, replacing leucine with serine, was identified within the N-terminal region of the… CONTINUE READING