Thermal unfolding transition of ribonuclease A measured by 2'-CMP binding.

@article{Nall1977ThermalUT,
  title={Thermal unfolding transition of ribonuclease A measured by 2'-CMP binding.},
  author={Barry T Nall and Robert L. Baldwin},
  journal={Biochemistry},
  year={1977},
  volume={16 16},
  pages={3572-6}
}
We report an approach to the problem of detecting and characterising intermediates in the unfolding of ribonuclease A. Two distinct properties of the protein are compared at equilibrium within the unfolding transition zone: (1) a physical property of the protein, the absorbance of buried tyrosine residues, and (2) a functional property, the ability to bind the specific ligand, 2'-CMP. A direct comparison of these two properties is made within the pH 5.8 transition zone, and an indirect… CONTINUE READING