Thermal unfolding of staphylococcal nuclease and several mutant forms thereof studied by differential scanning calorimetry.

@article{Tanaka1993ThermalUO,
  title={Thermal unfolding of staphylococcal nuclease and several mutant forms thereof studied by differential scanning calorimetry.},
  author={Akira Tanaka and John Flanagan and Julian M. Sturtevant},
  journal={Protein science : a publication of the Protein Society},
  year={1993},
  volume={2 4},
  pages={567-76}
}
The effects of eight mutations on the thermodynamics of the reversible thermal unfolding of staphylococcal nuclease have been determined over a range of pH and protein concentration by means of differential scanning calorimetry. Variation of the protein concentration was included in our study because we found a significant dependence of the thermodynamics of protein unfolding on concentration. Values for the change in the standard free energy of unfolding, delta delta G0d, produced by the… CONTINUE READING

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