Thermal unfolding and proteolytic susceptibility of ribonuclease A.

@article{Arnold1996ThermalUA,
  title={Thermal unfolding and proteolytic susceptibility of ribonuclease A.},
  author={Ulrich Arnold and Karl Peter Ruecknagel and Angelika Schierhorn and R Ulbrich-hofmann},
  journal={European journal of biochemistry},
  year={1996},
  volume={237 3},
  pages={862-9}
}
With the aim to localize the structural region that becomes first accessible to proteolytic attack during thermal unfolding, the proteolysis of ribonuclease A was studied in the temperature range of 20-65 degrees C. Subtilisin, proteinase K, and elastase proved to be not appropriate as indicators of thermal unfolding, because even the native protein molecule was cleaved by these proteases. In contrast, chymotrypsin, trypsin, and thermolysin attacked ribonuclease A only after its thermal… CONTINUE READING
11 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Similar Papers

Loading similar papers…