Thermal denaturation of proteins for SDS-PAGE analysis by microwave irradiation.

Abstract

One of the most useful tools available to researchers is sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (3). It is a rapid and relatively simple method for resolving proteins and is routinely used to monitor proteins during purification, assess expression levels, estimate molecular weights and characterize multimeric proteins. A number… (More)

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