Thermal and structural behavior of natural cerebroside 3-sulfate in bilayer membranes.

  title={Thermal and structural behavior of natural cerebroside 3-sulfate in bilayer membranes.},
  author={M. J. Ruocco and G. Shipley},
  journal={Biochimica et biophysica acta},
  volume={859 2},
Differential scanning calorimetry (DSC), polarizing microscopy and X-ray diffraction studies have been performed on dry and hydrated natural bovine brain sulfatides. Dry sulfatide fractions exhibit a high temperature transition (delta H = 6.6 kcal/mol sulfatide) at 87.3 degrees C. X-ray diffraction shows this transition to be associated with a hydrocarbon chain order-disorder transformation between two lamellar phases. Hydrated sulfatide dispersions undergo a complex chain order-disorder… Expand
Unusual hydration properties of C16:0 sulfatide bilayer membranes.
The continuous swelling behavior exhibited by the L(alpha) bilayer phase of C16:0-GalSulf is typical of lipids bearing a net negative charge and confirms that the presence of 100 mM Na(+) is insufficient to shield the charge contributed by the sulfate group. Expand
Bilayer properties of totally synthetic C16:0-lactosyl-ceramide.
Two thermal protocols were used to completely convert C16:0-LacCer to its stable, high melting temperature form, and over the temperature range 20-78 degrees C this stable phase exhibits a bilayer structure, periodicity d approximately 65 A with an ordered chain packing mode. Expand
Interdigitated lipid bilayers of long acyl species of cerebroside sulfate. An X-ray diffraction study
Abstract X-ray diffraction analysis was performed on two species of cerebroside sulfate containing lignoceric acid (C24-CBS) or hexacosanoic acid (C26-CBS) hydrated in the presence of Li + or K +Expand
Polymorphic phases of galactocerebrosides: spectroscopic evidence of lamellar crystalline structures.
Spectral changes indicated that the temperature and/or time induced formation of the lamellar crystalline structures of NFA- and HFA-GalCer was accompanied by partial dehydration and by rearrangements of the hydrogen bonding network and bilayer packing mode of GalCer. Expand
Phase Behavior and Lipid-Membrane Structure of Phospholipid-Glycosphingolipid Liposomes and the Thermal Unfolding of Insulin
AbstractThe thermodynamic phase behavior and lipid-membrane structure of fully hydrated uni- and multilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) surface-modified by aExpand
Properties of ganglioside GM1 in phosphatidylcholine bilayer membranes.
The thermotropic and structural properties of ganglioside GM1 alone and in a binary system with 1,2-dipalmitoyl phosphatidylcholine (DPPC) have been investigated by differential scanning calorimetry (DSC) and x-ray diffraction. Expand
The ordering and dynamics of the terminal methyl group on the lignoceric acid chain in cerebroside sulfate
Cerebroside sulfate containing lignoceric acid deuterated at the terminal methyl group was prepared by a semi-synthetic method in order to study the ordering and motion of the fatty acyl chain byExpand
Influence of structural modifications on the phase behavior of semi-synthetic cerebroside sulfate.
It could be concluded that fatty acid chain length has little effect on the phase transition temperature and enthalpy, which suggests that the forces between the lipid molecules may be dominated by head group interactions rather than interactions between the cholesterol chains. Expand
Probing the Double Bond and Phase Properties of Natural Lipid Dispersions by Cross Polarization/Magic Angle Spinning 13C NMR
Solid-state high resolution 13C NMR spectra of bovine brain sulfatide, egg sphingomyelin, egg phosphatidylcholine, and cholesterol/phosphatidylcholine dispersions were obtained by theExpand
Bilayer dimensions and hydration of glycolipids.
  • D. Marsh
  • Chemistry, Medicine
  • Chemistry and physics of lipids
  • 2012
The bilayer thickness and hence, from the lamellar repeat spacing, the water layer thickness and degree of hydration of the lipid polar groups are obtained by using the available data for bilayer-forming diacyl and dialkyl glycosylglycerols, and for certain glycosphingolipids. Expand


Galactocerebroside-phospholipid interactions in bilayer membranes.
Differential scanning calorimetry and x-ray diffraction have been used to study the interaction of hydrated N-palmitoylgalactosylsphingosine (NPGS) and dipalMIToylphosphatidylcholine (DPPC), finding complete miscibility of NPGS into hydrated DPPC bilayers is observed in both the bilayer gel and liquid-crystal phases. Expand
Calorimetric investigation of the complex phase behavior of glucocerebroside dispersions.
Results indicate that glucocerebroside undergoes two distinct phase transitions centered at 47 and 83 degrees C, respectively, which define a unidirectional cycle in which each state of the molecule can only be reached by completing the entire cycle. Expand
X-ray diffraction and calorimetric study of anhydrous and hydrated N-palmitoylgalactosylsphingosine (cerebroside).
It is suggested that hydrated crystal form E is stabilized by both a highly ordered chain packing mode and a lateral intermolecular hydrogen bonding network involving the sphingosine backbone, the galactosyl group, and interbilayer water molecules. Expand
Thermal behavior of fractionated and unfractionated bovine brain cerebrosides.
Systematic variation of cooling/heating protocols indicates that NFA-CER possesses two low-temperature states, one metastable and the other stable, and suggests that a function of hydroxy fatty acids in brain cerebrosides may be to prevent metastable dehydration in the cerebroside-rich myelin membrane. Expand
Structure des phases liquide-cristallines de différents phospholipides, monoglycérides, sphingolipides, anhydres ou en présence d'eau
Abstract X-Ray diffraction studies have been carried out on some lipids of various chemical compositions: two monoglycerides (glyceryl-monodecanoate and glyceryl-monododecanoate), severalExpand
Effect of fatty acid chain length, fatty acid hydroxylation, and various cations on phase behavior of synthetic cerebroside sulfate
Abstract Synthetic species of CBS containing palmitic, stearic, lignoceric, D-2-hydroxy palmitic, or D-2-hydroxy stearic acid were prepared and their phase behavior in the presence of a number ofExpand
Molecular arrangements in sphingolipids. The crystal structure of cerebroside
The single crystal analysis of a complex, membrane glycolipid is described. Cerebroside (β-D-galactosyl-N-(2-D-hydroxyoctadecanoyl)-D-dihydrosphingosine, C42H32O9N ·12C2H5OH) — an importantExpand
Partial synthesis and physical properties of cerebroside sulfate containing palmitic acid or alpha-hydroxy palmitic acid.
The thermotropic behavior of the two synthetic CBSs were compared to each other and to the corresponding components in natural CBS, in order to determine the contribution of the hydroxy fatty acid to intermolecular hydrogen bonding between molecules of the lipid. Expand
Molecular arrangements in glycosphingolipids.
Whereas in earlier surface film studies of complex lipids, only one liquid expanded phase usually has been found, cerebrosides also exhibit numerous condensed phases, and comparisons with corresponding natural lipids have shown a close relationship both in the phase behaviour and structure of the different polymorphs. Expand
Changes in the fatty acid composition of cerebrosides and sulfatides of human nervous tissue with age.
Fatty acid changes with age were the same for cerebrosides and sulfatide but occurred later in the sulfatides, which supports the hypothesis that the cerebroides are precursors of the sulf atides. Expand