Thermal Stability of the Plasma Membrane Calcium Pump. Quantitative Analysis of Its Dependence on Lipid-Protein Interactions

@article{Levi2000ThermalSO,
  title={Thermal Stability of the Plasma Membrane Calcium Pump. Quantitative Analysis of Its Dependence on Lipid-Protein Interactions},
  author={Valeria Levi and J. A. Dei Rossi and Mar{\'i}a Mercedes Echarte and Pablo Ra{\'u}l Castello and F. Luis Gonz{\'a}lez Flecha},
  journal={The Journal of Membrane Biology},
  year={2000},
  volume={173},
  pages={215-225}
}
Thermal stability of plasma membrane Ca2+ pump was systematically studied in three micellar systems of different composition, and related with the interactions amphiphile-protein measured by fluorescence resonance energy transfer. Thermal denaturation was characterized as an irreversible process that is well described by a first order kinetic with an activation energy of 222 ± 12 kJ/mol in the range 33–45°C. Upon increasing the mole fraction of phospholipid in the mixed micelles where the Ca2… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 38 references

The Hydrophobic effect: Formation of micelles

  • C. Tanford
  • 1980
Highly Influential
4 Excerpts

Molecular characterization of the glycated plasma membrane calcium pump

  • F. L. González Flecha, P. R. Castello, J. J. Gagliardino, J. P. Rossi
  • J . Membrane Biol .
  • 1999
1 Excerpt

A method for assessing the stability

  • Press, F. W. New York Lau, J. U. Bowie
  • 1997

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