Therapy for mucosal infections


The study of protein–protein interactions that involve integral membrane proteins has been thwarted by the difficulty of biochemical approaches and the unsuitability of genetic two-hybrid approaches. On page 1075, Ehrhard et al. describe a way of unraveling such interactions using a yeast genetic approach that exploits the G-protein signaling pathway. One binding partner is a soluble protein fused to the G-protein γ-subunit, and the other is an integral membrane protein. Interaction between the two binding partners disrupts the G-protein signaling pathway, leading to reduced transcription of a reporter gene and growth arrest. The authors validated the approach by detecting interactions between the fibroblast-derived growth factor receptor 3 and the cytoplasmic protein SNT-1, and went on to study mutations that disrupt interactions between the the neuronal proteins syntaxin 1a and and nSec1. JJ Electrocatalytic DNA mismatch detection

DOI: 10.1038/80195

Cite this paper

@article{Sinclair2000TherapyFM, title={Therapy for mucosal infections}, author={Meeghan L Sinclair}, journal={Nature Biotechnology}, year={2000}, volume={18}, pages={1027-1027} }