Therapeutic effect of dendritic cells loaded with a fusion mRNA encoding tyrosinase-related protein 2 and enhanced green fluorescence protein on B16 melanoma.

@article{Fukui2004TherapeuticEO,
  title={Therapeutic effect of dendritic cells loaded with a fusion mRNA encoding tyrosinase-related protein 2 and enhanced green fluorescence protein on B16 melanoma.},
  author={Mikiko Fukui and Tomoko Nakano-Hashimoto and Kozue Okano and Yuichiro Maruta and Yutaka Suehiro and Yuichiro Hamanaka and Hiroshi Yamashita and Kohzoh Imai and Michio M. Kawano and Yuji Hinoda},
  journal={Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine},
  year={2004},
  volume={25 5-6},
  pages={252-7}
}
Dendritic cells (DCs) loaded with messenger RNA (mRNA) have been proposed to be useful for inducing specific cytotoxic T lymphocytes against tumor antigens. It is now also apparent that tumor antigen-specific T cell tolerance limits the efficacy of active immunotherapy. To improve the efficacy of mRNA-loaded DCs, we constructed a fusion mRNA encoding tyrosinase-related protein 2 (TRP2), which has a late endosomal/lysosomal sorting signal and enhanced green fluorescence protein (EGFP), and… CONTINUE READING