Therapeutic drug monitoring for sirolimus in whole blood of organ transplants by high-performance liquid chromatography with ultraviolet detection.

@article{Campanero2004TherapeuticDM,
  title={Therapeutic drug monitoring for sirolimus in whole blood of organ transplants by high-performance liquid chromatography with ultraviolet detection.},
  author={Miguel Angel Campanero and Ernesto Cardenas and Bel{\'e}n S{\'a}daba and Emilio Garc{\'i}a-Quetglas and Maria Jos{\'e} Mu{\~n}oz-Juarez and Isabel Gil-Aldea and David Pazo and Jos{\'e} Ram{\'o}n Azanza and Jes{\'u}s Honorato},
  journal={Journal of chromatography. A},
  year={2004},
  volume={1031 1-2},
  pages={265-73}
}
We developed and validated an accurate, sensitive, precise and rapid HPLC method with UV detection for the determination of sirolimus in blood samples from renal, cardiac and hepatic transplants. This method overcomes most of the problems related to previously published assays using a narrow-bore column with base deactivated C18 reversed phase. Whole blood samples were purified by a combination of a precipitating blood matrix with zinc sulphate and a single step liquid-liquid extraction with… CONTINUE READING

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