Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging.

@article{Philip2003TheoreticalIO,
  title={Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging.},
  author={Johan Philip and Kjell Carlsson},
  journal={Journal of the Optical Society of America. A, Optics, image science, and vision},
  year={2003},
  volume={20 2},
  pages={368-79}
}
We deduce the signal-to-noise ratio for fluorescence lifetime imaging when using frequency-domain methods. We assume mono-exponential decay and quantum-noise-limited performance. The results are compared with Monte Carlo simulations with good agreement. We also compare our results with previous investigations of time-domain methods for fluorescence lifetime imaging. For a given number of detected photons, we find that frequency-domain and time-domain methods are equally good. The correct choice… CONTINUE READING

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