Theoretical considerations on mechanisms of harvesting cells cultured on thermoresponsive polymer brushes.

@article{Halperin2012TheoreticalCO,
  title={Theoretical considerations on mechanisms of harvesting cells cultured on thermoresponsive polymer brushes.},
  author={Avraham Halperin and Martin Kr{\"o}ger},
  journal={Biomaterials},
  year={2012},
  volume={33 20},
  pages={4975-87}
}
Poly (N-isopropylacrylamide) (PNIPAM) brushes and hydrogels serve as temperature-responsive cell culture substrates. The cells adhere at 37 °C and are detached by cooling to below the lower critical solution temperature T(LCST) ≈ 32 °C, an effect hitherto attributed to change in PNIPAM hydration. The article proposes a mechanism coupling the change of hydration to integrin mediated environmental sensing for cell culture on brushes and hydrogels in serum containing medium. Hydration is… CONTINUE READING

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PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion.

Colloids and surfaces. B, Biointerfaces • 2017
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