Theoretical basis of confocal microscopy.

  title={Theoretical basis of confocal microscopy.},
  author={Robert H. Webb},
  journal={Methods in enzymology},
A confocal microscope forms its image by recording light primarily from a small focal volume, largely ignoring points to the side or above or below. That volume, described as a point-spread function, is the product of two similar functions that are generated by the objective lens. Because of that multiplication, the recorded light is greater than even the… CONTINUE READING