The yeast vacuolar membrane proteome.

@article{Wiederhold2009TheYV,
  title={The yeast vacuolar membrane proteome.},
  author={Elena Wiederhold and T. Gandhi and Hjalmar P. Permentier and Rainer Breitling and Bert Poolman and Dirk Jan Slotboom},
  journal={Molecular & cellular proteomics : MCP},
  year={2009},
  volume={8 2},
  pages={
          380-92
        }
}
Transport of solutes between the cytosol and the vacuolar lumen is of crucial importance for various functions of vacuoles, including ion homeostasis; detoxification; storage of different molecules such as amino acids, phosphate, and calcium ions; and proteolysis. To identify proteins that catalyze solute transport across the vacuolar membrane, the membrane proteome of purified Saccharomyces cerevisiae vacuoles was analyzed. Subtractive proteomics was used to distinguish contaminants from true… CONTINUE READING

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