The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo.

@article{Duprs2009TheYW,
  title={The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo.},
  author={Vincent Dupr{\`e}s and David Alsteens and Sabrina Wilk and Benjamin H. Hansen and J{\"u}rgen J. Heinisch and Yves F Dufr{\^e}ne},
  journal={Nature chemical biology},
  year={2009},
  volume={5 11},
  pages={857-62}
}
Here we report on in vivo measurement of the mechanical behavior of a cell surface sensor using single-molecule atomic force microscopy. We focus on the yeast wall stress component sensor Wsc1, a plasma membrane protein that is thought to function as a rigid probe of the cell wall status. We first map the distribution of individual histidine-tagged sensors on living yeast cells by scanning the cell surface with atomic force microscopy tips carrying nitrilotriacetate groups. We then show that… CONTINUE READING
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