The winnowing: establishing the squid–vibrio symbiosis

  title={The winnowing: establishing the squid–vibrio symbiosis},
  author={Spencer V. Nyholm and Margaret J. McFall-Ngai},
  journal={Nature Reviews Microbiology},
Most symbiotic associations between animals and microorganisms are horizontally transmitted — the microorganisms are acquired from the environment by each generation of the host. How are exclusive partnerships established in the context of the thousands of other microbial species that are present in the environment? Similar to winnowing during a harvest, the symbiosis between the squid Euprymna scolopes and its luminous bacterial symbiont Vibrio fischeri involves a step-wise elimination of… 
Host-microbe symbiosis: the squid-Vibrio association--a naturally occurring, experimental model of animal/bacterial partnerships.
  • M. McFall-Ngai
  • Biology, Environmental Science
    Advances in experimental medicine and biology
  • 2008
In this chapter, the association between the Hawaiian sepiolid squid Euprymna scolopes and the marine luminous bacterium Vibrio fisheri is highlighted, offering a relatively simple, yet naturally occurring, association that can be experimentally manipulated.
Vibrio fischeri metabolism: symbiosis and beyond.
  • A. Dunn
  • Biology
    Advances in microbial physiology
  • 2012
Colonization of Euprymna scolopes squid by Vibrio fischeri.
A method to assess the degree of colonization that occurs when newly hatched aposymbiotic E. scolopes are exposed to (artificial) seawater containing V. fischeri and the Hawaiian bobtail squid is described.
The Mechanistic Benefits of Microbial Symbionts
  • C. J. Hurst
  • Biology
    Advances in Environmental Microbiology
  • 2016
This chapter will review how this highly specific association is established and maintained, highlighting the molecular mechanisms by which the partners communicate to achieve this goal and how the host’s innate immune system contributes to the specificity of the symbiosis.
Fiat Lux: The Squid–Vibrio Association as a Model for Understanding Host–Microbe Associations
The symbiosis between the Hawaiian bobtail squid Euprymna scolopes and the bioluminescent bacterium Vibrio fischeri offers an experimentally tractable model for understanding the role of beneficial
The genomic code: inferring Vibrionaceae niche specialization
The Vibrionaceae show a wide range of niche specialization, from free-living forms to those attached to biotic and abiotic surfaces, from symbionts to pathogens and from estuarine inhabitants to
Microbial experimental evolution as a novel research approach in the Vibrionaceae and squid-Vibrio symbiosis
Despite virtues provided by the Vibrionaceae and sepiolid squid-Vibrio symbiosis, these assets to evolutionary biology have yet to be fully utilized for microbial experimental evolution.
Global discovery of colonization determinants in the squid symbiont Vibrio fischeri
This study examines colonization of a simplified model in which the light organ of Euprymna scolopes squid is colonized exclusively by Vibrio fischeri bacteria, and applies high-throughput insertion sequencing to identify which bacterial genes are required during host colonization.


Consequences of evolving with bacterial symbionts : Insights from the squid-vibrio associations
▪ Abstract The squid-vibrio light-organ symbioses, which have been under investigation for just over 10 years, offer the opportunity to decipher aspects of the dynamics of stable associations between
Symbiont recognition and subsequent morphogenesis as early events in an animal-bacterial mutualism.
Bacterial colonization of the developing light organ of the squid Euprymna scolopes is shown to be highly specific, with the establishment of a successful association resulting only when the juvenile
The Euprymna scolopes-Vibrio fischeri symbiosis: a biomedical model for the study of bacterial colonization of animal tissue.
  • E. Ruby
  • Biology
    Journal of molecular microbiology and biotechnology
  • 1999
The association between the bioluminescent bacterium Vibrio fischeri and the sepiolid squid Euprymna scolopes has emerged as a productive model system for the investigation of the mechanisms by which cooperative bacteria initiate colonization of specific host tissues.
Population Dynamics of Vibrio fischeri during Infection of Euprymna scolopes
Results indicated that, when exposed to low numbers of V. fischeri, the host may become colonized by only one or a few bacterial cells, suggesting that symbiotic infection is highly efficient.
Sepiolids and Vibrios: When First They Meet
This work has shown that among those taxa that have been identified, a substantial number of microbial species have not been fully identified and are likely to be beneficial to animals and the environment.
Wolbachia: A Tale of Sex and Survival
By manipulating the sex lives of its hosts, the ubiquitous bacterium Wolbachia --perhaps the most common infectious bacterium on Earth--boosts its own reproductive success. Although no vertebrates
Dominance of Vibrio fischeri in Secreted Mucus outside the Light Organ of Euprymna scolopes: the First Site of Symbiont Specificity
Experiments with green fluorescent protein-labeled symbiotic and nonsymbiotic species of gram-negative bacteria were used to characterize the behavior of cells in the aggregates, providing evidence that the specificity of the squid-vibrio symbiosis begins early in the interaction, in the mucus where the symbionts aggregate outside of the light organ.
Growth and flagellation of Vibrio fischeri during initiation of the sepiolid squid light organ symbiosis
The kinetics of the process by which newly hatched juvenile squids become infected by symbiosis-competent V. fischeri are document here and it is shown that growth rate and flagellation were modulated during establishment of the association.
Effect of the Squid Host on the Abundance and Distribution of Symbiotic Vibrio fischeri in Nature
Findings constitute evidence for the first recognized instance of the abundance and distribution of a marine bacterium being driven primarily by its symbiotic association with an animal host.
The Vibrio fischeri–Euprymna scolopes Light Organ Symbiosis
The observation that lipopolysaccharide and peptidoglycan monomer stimulate developmental processes in a mutualistic animal-bacteria association is notable for at least two reasons, which suggests conserved mechanisms for mutualist and pathogen detection by animals, with host responses being context dependent.